European Ocean Biodiversity Information System

Project Data Monitoring in the Port of Trieste Title Port Authority - Autorità Portuale Identifier 1533 Study Area Description Trieste harbour, Northern Adriatic Sea

Sampling Methods Sampling was carried out by bottom corer. Study Extent Two monitoring campaigns were performed (June 2013 and March 2015). Quality Control Replicates were sampled and analysed to take into account biological variability. Method step description: Sampling was carried out in the harbour of the Trieste port by a KC Haps bottom corer with polycarbonate sample tubes (12.7 cm I.D. with a sample area of 127 cm2). In laboratory, one sediment core was subsampled by means of 3 cut-off plastic syringes (2.7 cm I.D., surface area of 5.72 cm2, 0-10 cm top sediment) in order to obtain three pseudoreplicates for the meiofauna. All data are converted to by multiplying them by a conversion factor (1.66). Meiofaunal organisms (38–1000 m) were extracted from sediment subsamples by sieving each pseudoreplicate and by centrifuging the material retained by a 38 μm mesh net with Ludox HS-40 (density of 1.15–1.18 g cm−3). All meiobenthic organisms were counted and taxonomically classified to the main groups under a stereomicroscope (Olympus SZX12; final magnification of × 32-40).