European Ocean Biodiversity Information System

This dataset contain the macrobenthic data collected in the subtidal area. Macrobenthos are the biota that lives in the sediment and are retained on a sieve of 1mm. The sampling design for the monitoring is following the BACI-design (Before After Control Impact), with a control area with similar habitat characteristics outside the influence of the suppletion activity. There is sampled before the suppletion and in the following two years after suppletion. In this BACI-monitoring, the seasonal aspect is taken into account, by taking samples in spring (March) and autumn (September). In the subtidal area, three depth strata were determined to investigated the influence of the suppletion outside the suppletion area. Stratum a (between low water line and -2m TAW) is the area were the foreshore suppletion is executed. Stratum b and c are taken respectively the depth zones -3m to -4m and -5m to -6m TAW. The amount of sampling locations was determined by the client and determines 15 points for each sampling moment. That amount of samples was via a systematic random stratified strategy distributed over each stratum (5 in each strata). The samples were taken with a Van Veen grab (0.1m²). The samples were sieved alive on a 1mm sieve and then fixed with an 8% formaldehyde-seawater solution. The sieved material is stained with eosin. All biota were determined and counted to species level following a standardized taxonomic discrimination protocol. This to determine the determination level and if certain life stages (larvae, juveniles) need to be discriminated. The biomass is determined as wet weight. All macrobenthic samples were taken following the ISO16665:2005(E)) (“Water quality – Guidelines for quantitative sampling and sample processing of marine soft-bottom macrofauna”). Moreover, the analyses (identification, counts and biomass) were conform NBN EN ISO/IEC 17025 standard executed (cfr. BELAC nr. 315-TEST: date 28/04/2016, valid until 05/03/2020).